Composite

Part:BBa_K594011:Design

Designed by: Yu Xiaoyan   Group: iGEM11_OUC-China   (2011-10-01)


A device that can accepts the 3--O-C6-HSL and then produces 3-O-C12-HSL and ECFP reporter.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1752
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1314
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1005


Design Notes

When ligate the RBS with others, 3A assembly is not as efficient as standard assembly.


Source

Besides the leuB from E.coli BL21 cloned with PCR by ourselves, all the other parts are from the iGEM, including F2622, K081009, B0034, E0030, B0015.

References